Synergy in monoclonal antibody neutralization of HIV-1 pseudoviruses and infectious molecular clones.

ABSTRACT: Early events in HIV infection are still poorly understood; virus derived from acute infections, the transmitted/founders IMCs, could provide more reliable information as they represent strains that established HIV infection in vivo, and therefore are investigated to elucidate potentially shared biological features.This study examined synergy in neutralization by six monoclonal antibodies targeting different domains in gp120 and gp41 and assayed in pairwise combination against 11 HIV-1 clade B strains, either Env pseudoviruses (PV, n?=?5) or transmitted/founder infectious molecular clones (T/F IMCs, n?=?6). Three of the early-infection env tested as PV were juxtaposed with T/F viruses derived from the same three patients, respectively.All antibodies reaching IC50 were assayed pairwise (n?=?50). T/F IMCs showed overall lower sensitivity to neutralization by single antibodies than PV, including within the three patient-matched pairs. Remarkably, combination index (CI) calculated using the Chow and Talalay method indicated synergy (CI?

SUBMITTER: Miglietta R 

PROVIDER: S-EPMC4274758 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature