Ontology highlight
ABSTRACT:
SUBMITTER: Zou Y
PROVIDER: S-EPMC4275151 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Zou Yi Y Xu Wei W Tsunematsu Yuta Y Tang Mancheng M Watanabe Kenji K Tang Yi Y
Organic letters 20141210 24
Biochemical studies of purified and dissected fungal polyketide synthase and nonribosomal peptide synthetase (PKS-NRPS) hybrid enzymes involved in biosynthesis of pseurotin and aspyridone indicate that one α-methylation step during polyketide synthesis is a prerequisite and a key checkpoint for chain transfer between PKS and NRPS modules. In the absence of the resulting γ-methyl feature, the completed polyketide intermediate is offloaded as an α-pyrone instead of being aminoacylated by the NRPS ...[more]