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Nuclear stiffening inhibits migration of invasive melanoma cells.

ABSTRACT: During metastasis, melanoma cells must be sufficiently deformable to squeeze through extracellular barriers with small pore sizes. We visualize and quantify deformability of single cells using micropipette aspiration and examine the migration potential of a population of melanoma cells using a flow migration apparatus. We artificially stiffen the nucleus with recombinant overexpression of ?50 lamin A, which is found in patients with Hutchison Gilford progeria syndrome and in aged individuals. Melanoma cells, both WM35 and Lu1205, both show reduced nuclear deformability and reduced cell invasion with the expression of ?50 lamin A. These studies suggest that cellular aging including expression of ?50 lamin A and nuclear stiffening may reduce the potential for metastatic cancer migration. Thus, the pathway of cancer metastasis may be kept in check by mechanical factors in addition to known chemical pathway regulation.

SUBMITTER: Ribeiro AJ

PROVIDER: S-EPMC4276563 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Nuclear stiffening inhibits migration of invasive melanoma cells.

Ribeiro Alexandre J S AJ Khanna Payal P Sukumar Aishwarya A Dong Cheng C Dahl Kris Noel KN

Cellular and molecular bioengineering 20141201 4

During metastasis, melanoma cells must be sufficiently deformable to squeeze through extracellular barriers with small pore sizes. We visualize and quantify deformability of single cells using micropipette aspiration and examine the migration potential of a population of melanoma cells using a flow migration apparatus. We artificially stiffen the nucleus with recombinant overexpression of Δ50 lamin A, which is found in patients with Hutchison Gilford progeria syndrome and in aged individuals. Me ...[more]

PMID: 25544862

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Dataset Information

Nuclear stiffening inhibits migration of invasive melanoma cells.

Publications

Nuclear stiffening inhibits migration of invasive melanoma cells.

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