Ontology highlight
ABSTRACT:
SUBMITTER: Paudel BP
PROVIDER: S-EPMC4277754 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Paudel Bishnu P BP Rueda David D
Journal of the American Chemical Society 20141121 48
All biological processes take place in highly crowded cellular environments. However, the effect that molecular crowding agents have on the folding and catalytic properties of RNA molecules remains largely unknown. Here, we have combined single-molecule fluorescence resonance energy transfer (smFRET) and bulk cleavage assays to determine the effect of a molecular crowding agents on the folding and catalysis of a model RNA enzyme, the hairpin ribozyme. Our single-molecule data reveal that PEG fav ...[more]