Ontology highlight
ABSTRACT:
SUBMITTER: Ji W
PROVIDER: S-EPMC4277763 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
ACS synthetic biology 20141206 12
In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. Here, we use conjugation, a natural DNA horizontal transfer process among bacterial species, to deliver an engineered CRISPR interference (CRISPRi) system for targeting specific genes in recipient Escherichia coli cells. We show that delivery of the CRISPRi s ...[more]