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High-efficiency detection of severe acute respiratory syndrome virus genetic material.

ABSTRACT: A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.

SUBMITTER: Weidmann M

PROVIDER: S-EPMC427811 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature

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High-efficiency detection of severe acute respiratory syndrome virus genetic material.

Weidmann Manfred M Zanotto Paolo M D A PM Weber Friedemann F Spiegel Martin M Brodt Hans Rheinhard HR Hufert Frank T FT

Journal of clinical microbiology 20040601 6

A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene. ...[more]

PMID: 15184466

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