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Ion channel probes for scanning ion conductance microscopy.

ABSTRACT: The sensitivity and selectivity of ion channels provide an appealing opportunity for sensor development. Here, we describe ion channel probes (ICPs), which consist of multiple ion channels reconstituted into lipid bilayers suspended across the opening of perflourinated glass micropipets. When incorporated with a scanning ion conductance microscope (SICM), ICPs displayed a distance-dependent current response that depended on the number of ion channels in the membrane. With distance-dependent current as feedback, probes were translated laterally, to demonstrate the possibility of imaging with ICPs. The ICP platform yields several potential advantages for SICM that will enable exciting opportunities for incorporation of chemical information into imaging and for high-resolution imaging.

SUBMITTER: Zhou Y

PROVIDER: S-EPMC4278694 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Ion channel probes for scanning ion conductance microscopy.

Zhou Yi Y Bright Leonard K LK Shi Wenqing W Aspinwall Craig A CA Baker Lane A LA

Langmuir : the ACS journal of surfaces and colloids 20141209 50

The sensitivity and selectivity of ion channels provide an appealing opportunity for sensor development. Here, we describe ion channel probes (ICPs), which consist of multiple ion channels reconstituted into lipid bilayers suspended across the opening of perflourinated glass micropipets. When incorporated with a scanning ion conductance microscope (SICM), ICPs displayed a distance-dependent current response that depended on the number of ion channels in the membrane. With distance-dependent curr ...[more]

PMID: 25425190

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