Establishment and characterization of immortalized bovine endometrial epithelial cells.
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ABSTRACT:
SUBMITTER: Bai H
PROVIDER: S-EPMC4282067 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Publications
Establishment and characterization of immortalized bovine endometrial epithelial cells.
Bai Hanako H Sakurai Toshihiro T Bai Rulan R Yamakoshi Sachiko S Aoki Etsunari E Kuse Mariko M Okuda Kiyoshi K Imakawa Kazuhiko K
Animal science journal = Nihon chikusan Gakkaiho 20140415 8
Bovine primary uterine endometrial epithelial cells (EECs) are not ideal for long-term studies, because primary EECs lose hormone responsiveness quickly, and/or they tend to have a short life span. The aims of this study were to establish immortalized bovine EECs and to characterize these cells following long-term cultures. Immortalized bovine EECs were established by transfecting retroviral vectors encoding human papillomavirus (HPV) E6 and E7, and human telomerase reverse transcriptase (hTERT) ...[more]
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