Unknown

Dataset Information

0

Specification of V? and V? usage by Tcra/Tcrd locus V gene segment promoters.

ABSTRACT: The Tcra/Tcrd locus undergoes V-D?-J? rearrangement in CD4(-)CD8(-) thymocytes to form the TCR? chain of the ?? TCR and V-J? rearrangement in CD4(+)CD8(+) thymocytes to form the TCR?-chain of the ?? TCR. Most V segments in the locus participate in V-J? rearrangement, but only a small and partially overlapping subset participates in V-D?-J? rearrangement. What specifies any particular Tcra/Tcrd locus V gene segment as a V?, a V?, or both is currently unknown. We tested the hypothesis that V segment usage is specified by V segment promoter-dependent chromatin accessibility in developing thymocytes. TRAV15/DV6 family V gene segments contribute to both the Tcrd and the Tcra repertoires, whereas TRAV12 family V gene segments contribute almost exclusively to the Tcra repertoire. To understand whether the TRAV15/DV6 promoter region specifies TRAV15/DV6 as a V?, we used gene targeting to replace the promoter region of a TRAV12 family member with one from a TRAV15/DV6 family member. The TRAV15/DV6 promoter region conferred increased germline transcription and histone modifications to TRAV12 in double-negative thymocytes and caused a substantial increase in usage of TRAV12 in Tcrd recombination events. Our results demonstrate that usage of TRAV15/DV6 family V gene segments for Tcrd recombination in double-negative thymocytes is regulated, at least in part, by intrinsic features of TRAV15/DV6 promoters, and argue that Tcra/Tcrd locus V? gene segments are defined by their local chromatin accessibility in CD4(-)CD8(-) thymocytes.

SUBMITTER: Naik AK 

PROVIDER: S-EPMC4282949 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Specification of Vδ and Vα usage by Tcra/Tcrd locus V gene segment promoters.

Naik Abani Kanta AK   Hawwari Abbas A   Krangel Michael S MS  

Journal of immunology (Baltimore, Md. : 1950) 20141203 2

The Tcra/Tcrd locus undergoes V-Dδ-Jδ rearrangement in CD4(-)CD8(-) thymocytes to form the TCRδ chain of the γδ TCR and V-Jα rearrangement in CD4(+)CD8(+) thymocytes to form the TCRα-chain of the αβ TCR. Most V segments in the locus participate in V-Jα rearrangement, but only a small and partially overlapping subset participates in V-Dδ-Jδ rearrangement. What specifies any particular Tcra/Tcrd locus V gene segment as a Vδ, a Vα, or both is currently unknown. We tested the hypothesis that V segme  ...[more]

Similar Datasets

Unknown Tcrd Rearrangement Redirects a Processive Tcra Recombination Program to Expand the Tcra Repertoire.
| S-EPMC5529038 | biostudies-literature
Unknown A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRδ and TCRα repertoires.
| S-EPMC4575630 | biostudies-literature
Transcriptomics An ectopic CTCF binding element disturbs Vδ usage at the mouse Tcra-Tcrd locus by regulating contact between Vδ and Dδ gene segments
2016-09-02 | E-GEOD-81351 | biostudies-arrayexpress
Unknown A role of the CTCF binding site at enhancer E? in the dynamic chromatin organization of the Tcra-Tcrd locus.
| S-EPMC7515734 | biostudies-literature
Other An ectopic CTCF binding element disturbs Vδ usage at the mouse Tcra-Tcrd locus by regulating contact between Vδ and Dδ gene segments
2016-09-02 | GSE81351 | GEO
Unknown Long-distance regulation of fetal V(?) gene segment TRDV4 by the Tcrd enhancer.
| S-EPMC3159822 | biostudies-literature
Transcriptomics A discrete chromatin loop in the murine Tcra/Tcrd locus shapes the TCRδ and TCRα repertoires
2015-08-10 | E-GEOD-67442 | biostudies-arrayexpress
Other A discrete chromatin loop in the murine Tcra/Tcrd locus shapes the TCRδ and TCRα repertoires
2015-08-10 | GSE67442 | GEO
Transcriptomics Tcrd rearrangement redirects a processive Tcra recombination program to expand the Tcra repertoire
2017-06-06 | GSE95826 | GEO
Transcriptomics Trav15-dv6 family Tcrd rearrangements diversify the Tcra repertoire
2021-10-21 | GSE186044 | GEO