Ontology highlight
ABSTRACT:
SUBMITTER: Filippone C
PROVIDER: S-EPMC4283219 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Filippone Claudia C Zhi Ning N Wong Susan S Lu Jun J Kajigaya Sachiko S Gallinella Giorgio G Kakkola Laura L Söderlund-Venermo Maria M Young Neal S NS Brown Kevin E KE
Virology 20080205 2
Three full-length genomic clones (pB19-M20, pB19-FL and pB19-HG1) of parvovirus B19 were produced in different laboratories. pB19-M20 was shown to produce infectious virus. To determine the differences in infectivity, all three plasmids were tested by transfection and infection assays. All three clones were similar in viral DNA replication, RNA transcription, and viral capsid protein production. However, only pB19-M20 and pB19-HG1 produced infectious virus. Comparison of viral sequences showed n ...[more]