Ontology highlight
ABSTRACT:
SUBMITTER: Chen CH
PROVIDER: S-EPMC4285349 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Chen Chih-Hong CH Namanja Andrew T AT Chen Yuan Y
Nature communications 20140929
Ubiquitin-like (Ubl) modifications regulate nearly all cellular functions in eukaryotes with the largest superfamily of Ubl-specific proteases being Cys proteases. SENP1 is a model for this protease family and responsible for processing SUMO. Here using nuclear magnetic resonance relaxation measurements, chemical shift perturbation and enzyme kinetic analysis, we provide structural insights into the mechanism of substrate recognition coupled enzymatic activation within SENP1. We find that residu ...[more]