Ontology highlight
ABSTRACT:
SUBMITTER: Warkocki Z
PROVIDER: S-EPMC4285774 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Warkocki Zbigniew Z Schneider Cornelius C Mozaffari-Jovin Sina S Schmitzová Jana J Höbartner Claudia C Fabrizio Patrizia P Lührmann Reinhard R
Genes & development 20150101 1
Structural rearrangement of the activated spliceosome (B(act)) to yield a catalytically active complex (B*) is mediated by the DEAH-box NTPase Prp2 in cooperation with the G-patch protein Spp2. However, how the energy of ATP hydrolysis by Prp2 is coupled to mechanical work and what role Spp2 plays in this process are unclear. Using a purified splicing system, we demonstrate that Spp2 is not required to recruit Prp2 to its bona fide binding site in the B(act) spliceosome. In the absence of Spp2, ...[more]