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Genome sequence of Coxiella burnetii strain Namibia.

ABSTRACT: We present the whole genome sequence and annotation of the Coxiella burnetii strain Namibia. This strain was isolated from an aborting goat in 1991 in Windhoek, Namibia. The plasmid type QpRS was confirmed in our work. Further genomic typing placed the strain into a unique genomic group. The genome sequence is 2,101,438 bp long and contains 1,979 protein-coding and 51 RNA genes, including one rRNA operon. To overcome the poor yield from cell culture systems, an additional DNA enrichment with whole genome amplification (WGA) methods was applied. We describe a bioinformatics pipeline for improved genome assembly including several filters with a special focus on WGA characteristics.

SUBMITTER: Walter MC

PROVIDER: S-EPMC4286197 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Genome sequence of Coxiella burnetii strain Namibia.

Walter Mathias C MC Öhrman Caroline C Myrtennäs Kerstin K Sjödin Andreas A Byström Mona M Larsson Pär P Macellaro Anna A Forsman Mats M Frangoulidis Dimitrios D

Standards in genomic sciences 20141229

We present the whole genome sequence and annotation of the Coxiella burnetii strain Namibia. This strain was isolated from an aborting goat in 1991 in Windhoek, Namibia. The plasmid type QpRS was confirmed in our work. Further genomic typing placed the strain into a unique genomic group. The genome sequence is 2,101,438 bp long and contains 1,979 protein-coding and 51 RNA genes, including one rRNA operon. To overcome the poor yield from cell culture systems, an additional DNA enrichment with who ...[more]

PMID: 25593636

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