Profiling of the Tox21 chemical collection for mitochondrial function to identify compounds that acutely decrease mitochondrial membrane potential.

ABSTRACT:

Background

Mitochondrial dysfunction has been implicated in the pathogenesis of a variety of disorders including cancer, diabetes, and neurodegenerative and cardiovascular diseases. Understanding whether different environmental chemicals and druglike molecules impact mitochondrial function represents an initial step in predicting exposure-related toxicity and defining a possible role for such compounds in the onset of various diseases.

Objectives

We sought to identify individual chemicals and general structural features associated with changes in mitochondrial membrane potential (MMP).

Methods

We used a multiplexed [two end points in one screen; MMP and adenosine triphosphate (ATP) content] quantitative high throughput screening (qHTS) approach combined with informati

SUBMITTER: Attene-Ramos MS 

PROVIDER: S-EPMC4286281 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature