Roles of PCNA ubiquitination and TLS polymerases ? and ? in the bypass of methyl methanesulfonate-induced DNA damage.

ABSTRACT: Translesion synthesis (TLS) provides a highly conserved mechanism that enables DNA synthesis on a damaged template. TLS is performed by specialized DNA polymerases of which polymerase (Pol) ? is important for the cellular response to DNA damage induced by benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), ultraviolet (UV) light and the alkylating agent methyl methanesulfonate (MMS). As TLS polymerases are intrinsically error-prone, tight regulation of their activity is required. One level of control is provided by ubiquitination of the homotrimeric DNA clamp PCNA at lysine residue 164 (PCNA-Ub). We here show that Pol? can function independently of PCNA modification and that Pol? can function as a backup during TLS of MMS-induced lesions. Compared to cell lines deficient for PCNA modification (Pcna(K164R)) or Pol?, double mutant cell lines display hypersensitivity to MMS but not to BPDE or UV-C. Double mutant cells also displayed delayed post-replicative TLS, accumulate higher levels of replication stress and delayed S-phase progression. Furthermore, we show that Pol? and Pol? are redundant in the DNA damage bypass of MMS-induced DNA damage. Taken together, we provide evidence for PCNA-Ub-independent activation of Pol? and establish Pol? as an important backup polymerase in the absence of Pol? in response to MMS-induced DNA damage.

SUBMITTER: Wit N 

PROVIDER: S-EPMC4288191 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature