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Nanoscale ligand spacing influences receptor triggering in T cells and NK cells.

ABSTRACT: Bioactive nanoscale arrays were constructed to ligate activating cell surface receptors on T cells (the CD3 component of the TCR complex) and natural killer (NK) cells (CD16). These arrays are formed from biofunctionalized gold nanospheres with controlled interparticle spacing in the range 25-104 nm. Responses to these nanoarrays were assessed using the extent of membrane-localized phosphotyrosine in T cells stimulated with CD3-binding nanoarrays and the size of cell contact area for NK cells stimulated with CD16-binding nanoarrays. In both cases, the strength of response decreased with increasing spacing, falling to background levels by 69 nm in the T cell/anti-CD3 system and 104 nm for the NK cell/anti-CD16 system. These results demonstrate that immune receptor triggering can be influenced by the nanoscale spatial organization of receptor/ligand interactions.

SUBMITTER: Delcassian D

PROVIDER: S-EPMC4288448 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Nanoscale ligand spacing influences receptor triggering in T cells and NK cells.

Delcassian Derfogail D Depoil David D Rudnicka Dominika D Liu Mengling M Davis Daniel M DM Dustin Michael L ML Dunlop Iain E IE

Nano letters 20131021 11

Bioactive nanoscale arrays were constructed to ligate activating cell surface receptors on T cells (the CD3 component of the TCR complex) and natural killer (NK) cells (CD16). These arrays are formed from biofunctionalized gold nanospheres with controlled interparticle spacing in the range 25-104 nm. Responses to these nanoarrays were assessed using the extent of membrane-localized phosphotyrosine in T cells stimulated with CD3-binding nanoarrays and the size of cell contact area for NK cells st ...[more]

PMID: 24125583

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