Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine arteritis virus.
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ABSTRACT:
SUBMITTER: Hans A
PROVIDER: S-EPMC4290937 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
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Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine arteritis virus.
Hans Aymeric A Gaudaire Delphine D Manuguerra Jean-Claude JC Leon Albertine A Gessain Antoine A Laugier Claire C Berthet Nicolas N Zientara Stephan S
Journal of clinical microbiology 20141022 1
This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization. ...[more]
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