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Integrator regulates transcriptional initiation and pause release following activation.

ABSTRACT: In unicellular organisms, initiation is the rate-limiting step in transcription; in metazoan organisms, the transition from initiation to productive elongation is also important. Here, we show that the RNA polymerase II (RNAPII)-associated multiprotein complex, Integrator, plays a critical role in both initiation and the release of paused RNAPII at immediate early genes (IEGs) following transcriptional activation by epidermal growth factor (EGF) in human cells. Integrator is recruited to the IEGs in a signal-dependent manner and is required to engage and recruit the super elongation complex (SEC) to EGF-responsive genes to allow release of paused RNAPII and productive transcription elongation.

SUBMITTER: Gardini A

PROVIDER: S-EPMC4292851 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Integrator regulates transcriptional initiation and pause release following activation.

Gardini Alessandro A Baillat David D Cesaroni Matteo M Hu Deqing D Marinis Jill M JM Wagner Eric J EJ Lazar Mitchell A MA Shilatifard Ali A Shiekhattar Ramin R

Molecular cell 20140904 1

In unicellular organisms, initiation is the rate-limiting step in transcription; in metazoan organisms, the transition from initiation to productive elongation is also important. Here, we show that the RNA polymerase II (RNAPII)-associated multiprotein complex, Integrator, plays a critical role in both initiation and the release of paused RNAPII at immediate early genes (IEGs) following transcriptional activation by epidermal growth factor (EGF) in human cells. Integrator is recruited to the IEG ...[more]

PMID: 25201415

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