Ontology highlight
ABSTRACT:
SUBMITTER: Bestul AJ
PROVIDER: S-EPMC4294675 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Bestul Andrew J AJ Christensen Jenna R JR Grzegorzewska Agnieszka P AP Burke Thomas A TA Sees Jennifer A JA Carroll Robert T RT Sirotkin Vladimir V Keenan Robert J RJ Kovar David R DR
Molecular biology of the cell 20141112 2
The evolutionarily conserved small actin-monomer binding protein profilin is believed to be a housekeeping factor that maintains a general pool of unassembled actin. However, despite similar primary sequences, structural folds, and affinities for G-actin and poly-L-proline, budding yeast profilin ScPFY fails to complement fission yeast profilin SpPRF temperature-sensitive mutant cdc3-124 cells. To identify profilin's essential properties, we built a combinatorial library of ScPFY variants contai ...[more]