Simple sequence repeat markers for the endangered species Clianthus puniceus and C. maximus (Fabaceae).
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ABSTRACT:
SUBMITTER: Houliston GJ
PROVIDER: S-EPMC4298236 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Publications
Simple sequence repeat markers for the endangered species Clianthus puniceus and C. maximus (Fabaceae).
Houliston Gary J GJ Ramón-Laca Ana A Jain Reema R Mitchell Caroline M CM Goeke Dagmar F DF
Applications in plant sciences 20150107 1
<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite markers were developed for Clianthus puniceus using a shotgun sequencing library and tested for cross amplification in the closely related C. maximus to inform population management of these two endangered species. •<h4>Methods and results</h4>We constructed a shotgun sequencing library using a Roche 454 sequencer and searched the resulting data set for putative microsatellite regions. We optimized 12 of these regions to produce poly ...[more]
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