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Resistance training preserves fat-free mass without impacting changes in protein metabolism after weight loss in older women.

ABSTRACT: This study assessed the effects of resistance training (RT) on energy restriction-induced changes in body composition, protein metabolism, and the fractional synthesis rate of mixed muscle proteins (FSRm) in postmenopausal, overweight women. Sixteen women (age 68 +/- 1 years, BMI 29 +/- 1 kg/m(2), mean +/- s.e.m.) completed a 16-week controlled diet study. Each woman consumed 1.0 g protein/kg/day. At baseline (weeks B1-B3) and poststudy (weeks RT12-RT13), energy intake matched each subject's need and during weeks RT1-RT11 was hypoenergetic by 2,092 kJ/day (500 kcal/day). From weeks RT1 to RT13, eight women performed RT 3 day/week (RT group) and eight women remained sedentary (SED group). RT did not influence the energy restriction-induced decrease in body mass (SED -5.8 +/- 0.6 kg; RT -5.0 +/- 0.2 kg) and fat mass (SED -4.1 +/- 0.9 kg; RT -4.7 +/- 0.5 kg). Fat free mass (FFM) and total body water decreased in SED (-1.6 +/- 0.4 and -2.1 +/- 0.5 kg) and were unchanged in RT (-0.3 +/- 0.4 and -0.4 +/- 0.7 kg) (group-by-time, P < or = 0.05 and P = 0.07, respectively). Protein-mineral mass did not change in either group (SED 0.4 +/- 0.2 kg; RT 0.1 +/- 0.4 kg). Nitrogen balance, positive at baseline (2.2 +/- 0.3 g N/day), was unchanged poststudy. After body mass loss, postabsorptive (PA) and postprandial (PP) leucine turnover, synthesis, and breakdown decreased. Leucine oxidation and balance were not changed. PA and total (PA + PP) FSRm in the vastus lateralis were higher after weight loss. RT did not influence these protein metabolism responses. In summary, RT helps older women preserve FFM during body mass loss. The comparable whole-body nitrogen retentions, leucine kinetics, and FSRm between groups are consistent with the lack of differential protein-mineral mass change.

SUBMITTER: Campbell WW

PROVIDER: S-EPMC4299870 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Resistance training preserves fat-free mass without impacting changes in protein metabolism after weight loss in older women.

Campbell Wayne W WW Haub Mark D MD Wolfe Robert R RR Ferrando Arny A AA Sullivan Dennis H DH Apolzan John W JW Iglay Heidi B HB

Obesity (Silver Spring, Md.) 20090226 7

This study assessed the effects of resistance training (RT) on energy restriction-induced changes in body composition, protein metabolism, and the fractional synthesis rate of mixed muscle proteins (FSRm) in postmenopausal, overweight women. Sixteen women (age 68 +/- 1 years, BMI 29 +/- 1 kg/m(2), mean +/- s.e.m.) completed a 16-week controlled diet study. Each woman consumed 1.0 g protein/kg/day. At baseline (weeks B1-B3) and poststudy (weeks RT12-RT13), energy intake matched each subject's nee ...[more]

PMID: 19247271

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Project description:Excessive adipose tissue mass underlies much of the metabolic health complications in obesity. Although exercise training is known to improve metabolic health in individuals with obesity, the effects of exercise training without weight loss on adipose tissue structure and metabolic function remain unclear. Thirty-six adults with obesity (body mass index = 33 ± 3 kg · m-2 ) were assigned to 12 weeks (4 days week-1 ) of either moderate-intensity continuous training (MICT; 70% maximal heart rate, 45 min; n = 17) or high-intensity interval training (HIIT; 90% maximal heart rate, 10 × 1 min; n = 19), maintaining their body weight throughout. Abdominal subcutaneous adipose tissue (aSAT) biopsy samples were collected once before and twice after training (1 day after last exercise and again 4 days later). Exercise training modified aSAT morphology (i.e. reduced fat cell size, increased collagen type 5a3, both P ≤ 0.05, increased capillary density, P = 0.05) and altered protein abundance of factors that regulate aSAT remodelling (i.e. reduced matrix metallopeptidase 9; P = 0.02; increased angiopoietin-2; P < 0.01). Exercise training also increased protein abundance of factors that regulate lipid metabolism (e.g. hormone sensitive lipase and fatty acid translocase; P ≤ 0.03) and key proteins involved in the mitogen-activated protein kinase pathway when measured the day after the last exercise session. However, most of these exercise-mediated changes were no longer significant 4 days after exercise. Importantly, MICT and HIIT induced remarkably similar adaptations in aSAT. Collectively, even in the absence of weight loss, 12 weeks of exercise training induced changes in aSAT structure, as well as factors that regulate metabolism and the inflammatory signal pathway in adults with obesity. KEY POINTS: Exercise training is well-known to improve metabolic health in obesity, although how exercise modifies the structure and metabolic function of adipose tissue, in the absence of weight loss, remains unclear. We report that both 12 weeks of moderate-intensity continuous training (MICT) and 12 weeks of high-intensity interval training (HIIT) induced modifications in adipose tissue structure and factors that regulate adipose tissue remodelling, metabolism and the inflammatory signal pathway in adults with obesity, even without weight loss (with no meaningful differences between MICT and HIIT). The modest modifications in adipose tissue structure in response to 12 weeks of MICT or HIIT did not lead to changes in the rate of fatty acid release from adipose tissue. These results expand our understanding about the effects of two commonly used exercise training prescriptions (MICT and HIIT) on adipose tissue remodelling that may lead to advanced strategies for improving metabolic health outcomes in adults with obesity.

Dataset Information

Resistance training preserves fat-free mass without impacting changes in protein metabolism after weight loss in older women.

Publications

Resistance training preserves fat-free mass without impacting changes in protein metabolism after weight loss in older women.

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