Lipopolysaccharide responsiveness in vocal fold fibroblasts.

ABSTRACT:

Background

Vocal fold fibroblast's (VFF) strategic location in the lamina propria and their ability to respond to external stimuli by producing inflammatory molecules suggest their possible direct involvement in innate immunity. Toll-like receptors (TLRs) are an essential signaling component to this response, as they allow for recognition of various microorganisms, leading to subsequent induction of pro-inflammatory genes. The objective of this study was to elucidate the role of VFF in the host immune response and subsequent influence on inflammatory cytokine secretion.

Methods

VFF derived from polyp, scar, and normal tissue were treated with 5 μg/ml lipopolysaccharide (LPS). TLR1 through 9, CD14, and MD-2 were measured during stable conditions by polymerase chain reaction (

SUBMITTER: King SN 

PROVIDER: S-EPMC4300178 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature