Efficient high-throughput resequencing of genomic DNA.
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ABSTRACT:
SUBMITTER: Miller RD
PROVIDER: S-EPMC430165 | biostudies-literature | 2003 Apr
REPOSITORIES: biostudies-literature
Publications
Efficient high-throughput resequencing of genomic DNA.
Miller Raymond D RD Duan Shenghui S Lovins Elizabeth G EG Kloss Ellen F EF Kwok Pui-Yan PY
Genome research 20030312 4
Targeted resequencing of genomic DNA from organisms such as humans is an important tool enabling experimental access to variation within the species and between similar species. Taking full advantage of the reference genome sequences in designing robust, specific PCR assays and using stringent conditions, resequencing can be done efficiently without purification of the PCR product. By using a 10-fold greater amount of one primer when setting up the PCR initially in a new version of asymmetric PC ...[more]
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