A general computational approach for repeat protein design.
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ABSTRACT:
SUBMITTER: Parmeggiani F
PROVIDER: S-EPMC4303030 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Publications
A general computational approach for repeat protein design.
Parmeggiani Fabio F Huang Po-Ssu PS Vorobiev Sergey S Xiao Rong R Park Keunwan K Caprari Silvia S Su Min M Seetharaman Jayaraman J Mao Lei L Janjua Haleema H Montelione Gaetano T GT Hunt John J Baker David D
Journal of molecular biology 20141114 2
Repeat proteins have considerable potential for use as modular binding reagents or biomaterials in biomedical and nanotechnology applications. Here we describe a general computational method for building idealized repeats that integrates available family sequences and structural information with Rosetta de novo protein design calculations. Idealized designs from six different repeat families were generated and experimentally characterized; 80% of the proteins were expressed and soluble and more ...[more]
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