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Distributed classifier based on genetically engineered bacterial cell cultures.


ABSTRACT: We describe a conceptual design of a distributed classifier formed by a population of genetically engineered microbial cells. The central idea is to create a complex classifier from a population of weak or simple classifiers. We create a master population of cells with randomized synthetic biosensor circuits that have a broad range of sensitivities toward chemical signals of interest that form the input vectors subject to classification. The randomized sensitivities are achieved by constructing a library of synthetic gene circuits with randomized control sequences (e.g., ribosome-binding sites) in the front element. The training procedure consists in reshaping of the master population in such a way that it collectively responds to the "positive" patterns of input signals by producing above-threshold output (e.g., fluorescent signal), and below-threshold output in case of the "negative" patterns. The population reshaping is achieved by presenting sequential examples and pruning the population using either graded selection/counterselection or by fluorescence-activated cell sorting (FACS). We demonstrate the feasibility of experimental implementation of such system computationally using a realistic model of the synthetic sensing gene circuits.

SUBMITTER: Didovyk A 

PROVIDER: S-EPMC4304444 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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Distributed classifier based on genetically engineered bacterial cell cultures.

Didovyk Andriy A   Kanakov Oleg I OI   Ivanchenko Mikhail V MV   Hasty Jeff J   Huerta Ramón R   Tsimring Lev L  

ACS synthetic biology 20141111 1


We describe a conceptual design of a distributed classifier formed by a population of genetically engineered microbial cells. The central idea is to create a complex classifier from a population of weak or simple classifiers. We create a master population of cells with randomized synthetic biosensor circuits that have a broad range of sensitivities toward chemical signals of interest that form the input vectors subject to classification. The randomized sensitivities are achieved by constructing  ...[more]

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