Unknown

Dataset Information

0

BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion.


ABSTRACT: We have previously demonstrated that the anti-apoptotic protein BAD is expressed in normal human breast tissue and shown that BAD inhibits expression of cyclin D1 to delay cell-cycle progression in breast cancer cells. Herein, expression of proteins in breast tissues was studied by immunohistochemistry and results were analyzed statistically to obtain semi-quantitative data. Biochemical and functional changes in BAD-overexpressing MCF7 breast cancer cells were evaluated using PCR, reporter assays, western blotting, ELISA and extracellular matrix invasion assays. Compared to normal tissues, Grade II breast cancers expressed low total/phosphorylated forms of BAD in both cytoplasmic and nuclear compartments. BAD overexpression decreased the expression of ?-catenin, Sp1, and phosphorylation of STATs. BAD inhibited Ras/MEK/ERK and JNK signaling pathways, without affecting the p38 signaling pathway. Expression of the metastasis-related proteins, MMP10, VEGF, SNAIL, CXCR4, E-cadherin and TlMP2 was regulated by BAD with concomitant inhibition of extracellular matrix invasion. Inhibition of BAD by siRNA increased invasion and Akt/p-Akt levels. Clinical data and the results herein suggest that in addition to the effect on apoptosis, BAD conveys anti-metastatic effects and is a valuable prognostic marker in breast cancer.

SUBMITTER: Cekanova M 

PROVIDER: S-EPMC4304679 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications


We have previously demonstrated that the anti-apoptotic protein BAD is expressed in normal human breast tissue and shown that BAD inhibits expression of cyclin D1 to delay cell-cycle progression in breast cancer cells. Herein, expression of proteins in breast tissues was studied by immunohistochemistry and results were analyzed statistically to obtain semi-quantitative data. Biochemical and functional changes in BAD-overexpressing MCF7 breast cancer cells were evaluated using PCR, reporter assay  ...[more]

Similar Datasets

| S-EPMC7798230 | biostudies-literature
| S-EPMC3865809 | biostudies-literature
| S-EPMC6349202 | biostudies-literature
| S-EPMC2687718 | biostudies-literature
| S-EPMC3204087 | biostudies-other
| S-EPMC3070685 | biostudies-literature
| S-EPMC6689663 | biostudies-literature
| S-EPMC4030585 | biostudies-literature
| S-EPMC3701000 | biostudies-literature
| S-EPMC3824537 | biostudies-other