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Nanostructured microfluidic digestion system for rapid high-performance proteolysis.


ABSTRACT: A novel microfluidic protein digestion system with a nanostructured and bioactive inner surface was constructed by an easy biomimetic self-assembly strategy for rapid and effective proteolysis in 2 minutes, which is faster than the conventional overnight digestion methods. It is expected that this work would contribute to rapid online digestion in future high-throughput proteomics.

SUBMITTER: Cheng G 

PROVIDER: S-EPMC4304898 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Nanostructured microfluidic digestion system for rapid high-performance proteolysis.

Cheng Gong G   Hao Si-Jie SJ   Yu Xu X   Zheng Si-Yang SY  

Lab on a chip 20150201 3


A novel microfluidic protein digestion system with a nanostructured and bioactive inner surface was constructed by an easy biomimetic self-assembly strategy for rapid and effective proteolysis in 2 minutes, which is faster than the conventional overnight digestion methods. It is expected that this work would contribute to rapid online digestion in future high-throughput proteomics. ...[more]

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