Unknown

Dataset Information

0

A surface plasmon resonance approach to monitor toxin interactions with an isolated voltage-gated sodium channel paddle motif.


ABSTRACT: Animal toxins that inhibit voltage-gated sodium (Na(v)) channel fast inactivation can do so through an interaction with the S3b-S4 helix-turn-helix region, or paddle motif, located in the domain IV voltage sensor. Here, we used surface plasmon resonance (SPR), an optical approach that uses polarized light to measure the refractive index near a sensor surface to which a molecule of interest is attached, to analyze interactions between the isolated domain IV paddle and Na(v) channel-selective ?-scorpion toxins. Our SPR analyses showed that the domain IV paddle can be removed from the Na(v) channel and immobilized on sensor chips, and suggest that the isolated motif remains susceptible to animal toxins that target the domain IV voltage sensor. As such, our results uncover the inherent pharmacological sensitivities of the isolated domain IV paddle motif, which may be exploited to develop a label-free SPR approach for discovering ligands that target this region.

SUBMITTER: Martin-Eauclaire MF 

PROVIDER: S-EPMC4306711 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

A surface plasmon resonance approach to monitor toxin interactions with an isolated voltage-gated sodium channel paddle motif.

Martin-Eauclaire Marie-France MF   Ferracci Géraldine G   Bosmans Frank F   Bougis Pierre E PE  

The Journal of general physiology 20150201 2


Animal toxins that inhibit voltage-gated sodium (Na(v)) channel fast inactivation can do so through an interaction with the S3b-S4 helix-turn-helix region, or paddle motif, located in the domain IV voltage sensor. Here, we used surface plasmon resonance (SPR), an optical approach that uses polarized light to measure the refractive index near a sensor surface to which a molecule of interest is attached, to analyze interactions between the isolated domain IV paddle and Na(v) channel-selective α-sc  ...[more]

Similar Datasets

2022-06-09 | GSE186729 | GEO
| S-EPMC4172297 | biostudies-literature
2021-07-13 | GSE179818 | GEO
| S-EPMC3972499 | biostudies-literature
| S-EPMC8740163 | biostudies-literature
| S-EPMC6338345 | biostudies-literature
| S-EPMC3266868 | biostudies-literature
2020-05-09 | GSE150134 | GEO
2020-02-08 | GSE136927 | GEO
| S-EPMC6775510 | biostudies-literature