Generation of L cells in mouse and human small intestine organoids.
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ABSTRACT:
SUBMITTER: Petersen N
PROVIDER: S-EPMC4306716 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Publications
Generation of L cells in mouse and human small intestine organoids.
Petersen Natalia N Reimann Frank F Bartfeld Sina S Farin Henner F HF Ringnalda Femke C FC Vries Robert G J RG van den Brink Stieneke S Clevers Hans H Gribble Fiona M FM de Koning Eelco J P EJ
Diabetes 20131015 2
Upon a nutrient challenge, L cells produce glucagon-like peptide 1 (GLP-1), a powerful stimulant of insulin release. Strategies to augment endogenous GLP-1 production include promoting L-cell differentiation and increasing L-cell number. Here we present a novel in vitro platform to generate functional L cells from three-dimensional cultures of mouse and human intestinal crypts. We show that short-chain fatty acids selectively increase the number of L cells, resulting in an elevation of GLP-1 rel ...[more]
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