Ontology highlight
ABSTRACT:
SUBMITTER: McClelland KS
PROVIDER: S-EPMC4309589 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
PloS one 20150128 1
Traditional gene targeting methods in mice are complex and time consuming, especially when conditional deletion methods are required. Here, we describe a novel technique for assessing gene function by injection of modified antisense morpholino oligonucleotides (MOs) into the heart of mid-gestation mouse embryos. After allowing MOs to circulate through the embryonic vasculature, target tissues were explanted, cultured and analysed for expression of key markers. We established proof-of-principle b ...[more]