Dataset Information

Impact of a high-fat meal on assessment of clopidogrel-induced platelet inhibition in healthy subjects.

ABSTRACT: BACKGROUND:Ideal conditions for platelet reactivity testing are critical for optimal selection of a P2Y12 inhibitor. Data are inconsistent regarding the impact of high-fat meals on test assessment. METHODS:Participants included 12 healthy subjects not taking antiplatelet drugs after a 12-hour fast. After baseline assessment, subjects were given a 600 mg dose of clopidogrel. Four hours later, maximum platelet inhibition was tested in the fasting state by light transmission aggregometry (LTA), VerifyNow P2Y12, vasodilator-stimulated phosphoprotein (VASP), and whole blood aggregometry (WBA). Subjects were then provided a high-fat meal, and platelet function was evaluated two hours later. Change in measured platelet aggregation by LTA was the primary endpoint of the study. The Wilcoxon Rank Sum test was used to compare the change in platelet reactivity between fasting and non-fasting conditions. The Spearman rho (?) correlation coefficient was used to evaluate the association between fasting platelet reactivity and the change following a high-fat meal. RESULTS:No significant change occurred in maximal light transmission, as assessed by LTA with 5 ?M ADP (p?=?0.15) and with 20 ?M ADP (p?=?0.07). There was a significant change in the area under the curve with 5 ?M ADP (p?=?0.03) but not with 20 ?M ADP (p?=?0.18). Although there was no significant change with the VerifyNow P2Y12 assay (p?=?0.16), the change was correlated with the initial fasting value (Spearman's rho p?=?0.008). The VASP assay and WBA varied minimally. CONCLUSION:The high-fat meal did not significantly alter platelet function assessment of commonly used platelet function tests. Greater intra-subject variability existed for the optically-dependent compared with non-optically dependent tests. TRIAL REGISTRATION:NCT01307657.

SUBMITTER: Dobesh PP

PROVIDER: S-EPMC4312467 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Impact of a high-fat meal on assessment of clopidogrel-induced platelet inhibition in healthy subjects.

Dobesh Paul P PP Urban Jamela F JF Shurmur Scott W SW Oestreich Julie H JH

Thrombosis journal 20150123 1

<h4>Background</h4>Ideal conditions for platelet reactivity testing are critical for optimal selection of a P2Y12 inhibitor. Data are inconsistent regarding the impact of high-fat meals on test assessment.<h4>Methods</h4>Participants included 12 healthy subjects not taking antiplatelet drugs after a 12-hour fast. After baseline assessment, subjects were given a 600 mg dose of clopidogrel. Four hours later, maximum platelet inhibition was tested in the fasting state by light transmission aggregom ...[more]

PMID: 25642145

Similar Datasets

Project description:BackgroundDyslipidemia is a feature of impaired metabolic health in conjunction with impaired glucose metabolism and central obesity. However, the contribution of factors to postprandial lipemia in healthy but metabolically at-risk adults is not well understood. We investigated the collective contribution of several physiologic and lifestyle factors to postprandial triglyceride (TG) response to a high-fat meal in healthy, overweight and obese adults.MethodsOverweight and obese adults (n = 35) underwent a high-fat meal challenge with blood sampled at fasting and hourly in the 4-hour postprandial period after a breakfast containing 50 g fat. Incremental area under the curve (iAUC) and postprandial magnitude for TG were calculated and data analyzed using a linear model with physiologic and lifestyle characteristics as explanatory variables. Model reduction was used to assess which explanatory variables contributed most to the postprandial TG response.ResultsTG responses to a high-fat meal were variable between individuals, with approximately 57 % of participants exceeded the nonfasting threshold for hypertriglyceridemia. Visceral adiposity was the strongest predictor of TG iAUC (β = 0.53, p = 0.01), followed by aerobic exercise frequency (β = 0.31, p = 0.05), insulin resistance based on HOMA-IR (β = 0.30, p = 0.04), and relative exercise intensity at which substrate utilization crossover occurred (β = 0.05, p = 0.04). For postprandial TG magnitude, visceral adiposity was a strong predictor (β = 0.43, p < 0.001) followed by aerobic exercise frequency (β = 0.23, p = 0.01), and exercise intensity for substrate utilization crossover (β = 0.53, p = 0.01).ConclusionsPostprandial TG responses to a high-fat meal was partially explained by several physiologic and lifestyle characteristics, including visceral adiposity, insulin resistance, aerobic exercise frequency, and relative substrate utilization crossover during exercise.Trial registrationClinicalTrials.gov, NCT04128839 , Registered 16 October 2019 - Retrospectively registered.

Dataset Information

Impact of a high-fat meal on assessment of clopidogrel-induced platelet inhibition in healthy subjects.

Publications

Impact of a high-fat meal on assessment of clopidogrel-induced platelet inhibition in healthy subjects.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets