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Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species.

ABSTRACT: We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.

SUBMITTER: Qurollo BA 

PROVIDER: S-EPMC4313244 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species.

Qurollo Barbara A BA   Riggins Dana D   Comyn Alaire A   Zewde Merone T MT   Breitschwerdt Edward B EB  

Journal of clinical microbiology 20140820 11

We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp. ...[more]

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