Ontology highlight
ABSTRACT:
SUBMITTER: Paul R
PROVIDER: S-EPMC4313564 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Paul Rakesh R Greenberg Marc M MM
Journal of the American Chemical Society 20150112 2
C2'-Nucleotide radicals have been proposed as key intermediates in direct strand break formation in RNA exposed to ionizing radiation. Uridin-2'-yl radical (1) was independently generated in single- and double-stranded RNA via photolysis of a ketone precursor. Direct stand breaks result from heterolytic cleavage of the adjacent C3'-carbon-oxygen bond. Trapping of 1 by O2 or β-mercaptoethanol (1 M) does not compete with strand scission, indicating that phosphate elimination is >10(6) s(-1). Uraci ...[more]