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MiR-19b regulates hTERT mRNA expression through targeting PITX1 mRNA in melanoma cells.


ABSTRACT: Human telomerase reverse transcriptase (hTERT) plays a crucial role in cancer development. We previously identified paired-like homeodomain1 (PITX1) as an hTERT suppressor gene. However, the underlying mechanisms that are involved in the regulation of PITX1 remain unknown. Here, we report that the microRNA-19b (miR-19b) regulates hTERT expression and cell proliferation through inhibition of PITX1. Compared with normal melanocyte cells, miR-19b expression was higher in most melanoma cells and was accompanied by downregulation of PITX1. Moreover, overexpression of miR-19b inhibited PITX1 mRNA translation through a miR-19b binding site within the 3'UTR of the PITX1 mRNA. Our combined findings indicate the participation of miR-19b as a novel upstream effector of hTERT transcription via direct targeting of PITX1.

SUBMITTER: Ohira T 

PROVIDER: S-EPMC4314654 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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miR-19b regulates hTERT mRNA expression through targeting PITX1 mRNA in melanoma cells.

Ohira Takahito T   Naohiro Sunamura S   Nakayama Yuji Y   Osaki Mitsuhiko M   Okada Futoshi F   Oshimura Mitsuo M   Kugoh Hiroyuki H  

Scientific reports 20150203


Human telomerase reverse transcriptase (hTERT) plays a crucial role in cancer development. We previously identified paired-like homeodomain1 (PITX1) as an hTERT suppressor gene. However, the underlying mechanisms that are involved in the regulation of PITX1 remain unknown. Here, we report that the microRNA-19b (miR-19b) regulates hTERT expression and cell proliferation through inhibition of PITX1. Compared with normal melanocyte cells, miR-19b expression was higher in most melanoma cells and was  ...[more]

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