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Genes transactivated by hepatitis C virus core protein, a microarray assay.


ABSTRACT: AIM:To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection. METHODS:Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods. RESULTS:The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method. CONCLUSION:HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes.

SUBMITTER: Liu M 

PROVIDER: S-EPMC4315987 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature

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Genes transactivated by hepatitis C virus core protein, a microarray assay.

Liu Min M   Zhang Shu-Lin SL   Cheng Jun J   Liu Yan Y   Wang Lin L   Shao Qing Q   Zhang Jian J   Lin Shu-Mei SM  

World journal of gastroenterology 20050601 22


<h4>Aim</h4>To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.<h4>Methods</h4>Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.<h4>Results</h4>The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was p  ...[more]

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