Ontology highlight
ABSTRACT:
SUBMITTER: Yan Q
PROVIDER: S-EPMC4318720 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Organic & biomolecular chemistry 20150201 7
Agonist-promoted G-protein coupled receptor (GPCR) endocytosis and recycling plays an important role in many signaling events in the cell. However, the approaches that allow fast and quantitative analysis of such processes still remain limited. Here we report an improved labeling approach based on the genetic fusion of a fluorogen activating protein (FAP) to a GPCR and binding of a sulfonated analog of the malachite green (MG) fluorogen to rapidly and selectively label cell surface receptors. Fl ...[more]