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Talbot holographic illumination nonscanning (THIN) fluorescence microscopy.

ABSTRACT: Optical sectioning techniques offer the ability to acquire three-dimensional information from various organ tissues by discriminating between the desired in-focus and out-of-focus (background) signals. Alternative techniques to confocal, such as active structured illumination, exist for fast optically sectioned images, but they require individual axial planes to be imaged consecutively. In this article, an imaging technique (THIN), by utilizing active Talbot illumination in 3D and multiplexed holographic Bragg filters for depth discrimination, is demonstrated for imaging in vivo 3D biopsy without mechanical or optical axial scanning.

SUBMITTER: Luo Y

PROVIDER: S-EPMC4321697 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Talbot holographic illumination nonscanning (THIN) fluorescence microscopy.

Luo Yuan Y Singh Vijay Raj VR Bhattacharya Dipanjan D Yew Elijah Y S EYS Tsai Jui-Chang JC Yu Sung-Liang SL Chen Hsi-Hsun HH Wong Jau-Min JM Matsudaira Paul P So Peter T C PTC Barbastathis George G

Laser & photonics reviews 20140808 5

Optical sectioning techniques offer the ability to acquire three-dimensional information from various organ tissues by discriminating between the desired in-focus and out-of-focus (background) signals. Alternative techniques to confocal, such as active structured illumination, exist for fast optically sectioned images, but they require individual axial planes to be imaged consecutively. In this article, an imaging technique (THIN), by utilizing active Talbot illumination in 3D and multiplexed ho ...[more]

PMID: 25678936

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