Ontology highlight
ABSTRACT:
SUBMITTER: Tanaka T
PROVIDER: S-EPMC4322369 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Tanaka Taku T Yokoi Tadashi T Tamalu Fuminobu F Watanabe Shu-Ichi S Nishina Sachiko S Azuma Noriyuki N
Scientific reports 20150210
We generated self-induced retinal ganglion cells (RGCs) with functional axons from human induced pluripotent stem cells. After development of the optic vesicle from the induced stem cell embryoid body in three-dimensional culture, conversion to two-dimensional culture, achieved by supplementation with BDNF, resulted in differentiation of RGCs at a rate of nearly 90% as indicated by a marginal subregion of an extruded clump of cells, suggesting the formation of an optic vesicle. Axons extended ra ...[more]