Ontology highlight
ABSTRACT:
SUBMITTER: Miller CN
PROVIDER: S-EPMC4322802 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Miller Cheryl N CN Steele Shaun P SP Brunton Jason C JC Jenkins Ronald J RJ LoVullo Eric D ED Taft-Benz Sharon A SA Romanchuk Artur A Jones Corbin D CD Dotson Garry D GD Collins Edward J EJ Kawula Thomas H TH
BMC microbiology 20141231
<h4>Background</h4>Francisella tularensis is a Gram-negative bacterium that infects hundreds of species including humans, and has evolved to grow efficiently within a plethora of cell types. RipA is a conserved membrane protein of F. tularensis, which is required for growth inside host cells. As a means to determine RipA function we isolated and mapped independent extragenic suppressor mutants in ∆ripA that restored growth in host cells. Each suppressor mutation mapped to one of two essential ge ...[more]