Ontology highlight
ABSTRACT:
SUBMITTER: Parker SJ
PROVIDER: S-EPMC4326228 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Parker Sarah J SJ Halligan Brian D BD Halligan Brian D BD Greene Andrew S AS
Proteomics 20100401 7
We report a new quantitative proteomics approach that combines the best aspects of stable isotope labeling of amino acids in cell culture (SILAC) labeling and spectral counting. The SILAC peptide count ratio analysis (SPeCtRA, http://proteomics.mcw.edu/visualize) method relies on MS(2) spectra rather than ion chromatograms for quantitation and therefore does not require the use of high mass accuracy mass spectrometers. The inclusion of a stable isotope label allows the samples to be combined bef ...[more]