DNA motion induced by electrokinetic flow near an Au coated nanopore surface as voltage controlled gate.

ABSTRACT: We used fluorescence microscopy to investigate the diffusion and drift motion of λ DNA molecules on an Au-coated membrane surface near nanopores, prior to their translocation through solid-state nanopores. With the capability of controlling electric potential at the Au surface as a gate voltage, Vgate, the motions of DNA molecules, which are presumably generated by electrokinetic flow, vary dramatically near the nanopores in our observations. We carefully investigate these DNA motions with different values of Vgate in order to alter the densities and polarities of the counterions, which are expected to change the flow speed or direction, respectively. Depending on Vgate, our observations have revealed the critical distance from a nanopore for DNA molecules to be attracted or repelled-DNA's

SUBMITTER: Sugimoto M 

PROVIDER: S-EPMC4326562 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature