Ontology highlight
ABSTRACT:
SUBMITTER: Lee JY
PROVIDER: S-EPMC4326837 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Lee Ju Yeon JY Lee Jaehyoun J Yue Hongjun H Lee Tae-Hee TH
The Journal of biological chemistry 20141229 7
The nucleosome is the fundamental packing unit of the eukaryotic genome, and CpG methylation is an epigenetic modification associated with gene repression and silencing. We investigated nucleosome assembly mediated by histone chaperone Nap1 and the effects of CpG methylation based on three-color single molecule FRET measurements, which enabled direct monitoring of histone binding in the context of DNA wrapping. According to our observation, (H3-H4)2 tetramer incorporation must precede H2A-H2B di ...[more]