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Active endocannabinoids are secreted on extracellular membrane vesicles.

ABSTRACT: Endocannabinoids primarily influence neuronal synaptic communication within the nervous system. To exert their function, endocannabinoids need to travel across the intercellular space. However, how hydrophobic endocannabinoids cross cell membranes and move extracellularly remains an unresolved problem. Here, we show that endocannabinoids are secreted through extracellular membrane vesicles produced by microglial cells. We demonstrate that microglial extracellular vesicles carry on their surface N-arachidonoylethanolamine (AEA), which is able to stimulate type-1 cannabinoid receptors (CB1), and inhibit presynaptic transmission, in target GABAergic neurons. This is the first demonstration of a functional role of extracellular vesicular transport of endocannabinoids.

SUBMITTER: Gabrielli M

PROVIDER: S-EPMC4328748 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Active endocannabinoids are secreted on extracellular membrane vesicles.

Gabrielli Martina M Battista Natalia N Riganti Loredana L Prada Ilaria I Antonucci Flavia F Cantone Laura L Matteoli Michela M Maccarrone Mauro M Verderio Claudia C

EMBO reports 20150107 2

Endocannabinoids primarily influence neuronal synaptic communication within the nervous system. To exert their function, endocannabinoids need to travel across the intercellular space. However, how hydrophobic endocannabinoids cross cell membranes and move extracellularly remains an unresolved problem. Here, we show that endocannabinoids are secreted through extracellular membrane vesicles produced by microglial cells. We demonstrate that microglial extracellular vesicles carry on their surface ...[more]

PMID: 25568329

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