Ontology highlight
ABSTRACT:
SUBMITTER: Diaz AA
PROVIDER: S-EPMC4330337 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Diaz Aaron A AA Qin Han H Ramalho-Santos Miguel M Song Jun S JS
Nucleic acids research 20141126 3
Genetic screens of an unprecedented scale have recently been made possible by the availability of high-complexity libraries of synthetic oligonucleotides designed to mediate either gene knockdown or gene knockout, coupled with next-generation sequencing. However, several sources of random noise and statistical biases complicate the interpretation of the resulting high-throughput data. We developed HiTSelect, a comprehensive analysis pipeline for rigorously selecting screen hits and identifying f ...[more]