Ontology highlight
ABSTRACT:
SUBMITTER: Johnson HE
PROVIDER: S-EPMC4332254 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Johnson Heath E HE King Samantha J SJ Asokan Sreeja B SB Rotty Jeremy D JD Bear James E JE Haugh Jason M JM
The Journal of cell biology 20150209 4
Mesenchymal cells such as fibroblasts are weakly polarized and reorient directionality by a lamellipodial branching mechanism that is stabilized by phosphoinositide 3-kinase (PI3K) signaling. However, the mechanisms by which new lamellipodia are initiated and directed are unknown. Using total internal reflection fluorescence microscopy to monitor cytoskeletal and signaling dynamics in migrating cells, we show that peripheral F-actin bundles/filopodia containing fascin-1 serve as templates for fo ...[more]