Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differences.

ABSTRACT: The human gut contains dense and diverse microbial communities which have profound influences on human health. Gaining meaningful insights into these communities requires provision of high quality microbial nucleic acids from human fecal samples, as well as an understanding of the sources of variation and their impacts on the experimental model. We present here a systematic analysis of commonly used microbial DNA extraction methods, and identify significant sources of variation. Five extraction methods (Human Microbiome Project protocol, MoBio PowerSoil DNA Isolation Kit, QIAamp DNA Stool Mini Kit, ZR Fecal DNA MiniPrep, phenol:chloroform-based DNA isolation) were evaluated based on the following criteria: DNA yield, quality and integrity, and microbial community structure based on Illumin

SUBMITTER: Wagner Mackenzie B 

PROVIDER: S-EPMC4332372 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature