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Bactobolin A binds to a site on the 70S ribosome distinct from previously seen antibiotics.

ABSTRACT: The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.

SUBMITTER: Amunts A

PROVIDER: S-EPMC4332689 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Bactobolin A binds to a site on the 70S ribosome distinct from previously seen antibiotics.

Amunts Alexey A Fiedorczuk Karol K Truong Thao T TT Chandler Josephine J Greenberg E Peter EP Ramakrishnan V V

Journal of molecular biology 20150103 4

The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations. ...[more]

PMID: 25562208

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