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The mitochondrial dicarboxylate and 2-oxoglutarate carriers do not transport glutathione.

ABSTRACT: Glutathione carries out vital protective roles within mitochondria, but is synthesised in the cytosol. Previous studies have suggested that the mitochondrial dicarboxylate and 2-oxoglutarate carriers were responsible for glutathione uptake. We set out to characterise the putative glutathione transport by using fused membrane vesicles of Lactococcus lactis overexpressing the dicarboxylate and 2-oxoglutarate carriers. Although transport of the canonical substrates could be measured readily, an excess of glutathione did not compete for substrate uptake nor could transport of glutathione be measured directly. Thus these mitochondrial carriers do not transport glutathione and the identity of the mitochondrial glutathione transporter remains unknown.

SUBMITTER: Booty LM

PROVIDER: S-EPMC4332691 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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The mitochondrial dicarboxylate and 2-oxoglutarate carriers do not transport glutathione.

Booty Lee M LM King Martin S MS Thangaratnarajah Chancievan C Majd Homa H James Andrew M AM Kunji Edmund R S ER Murphy Michael P MP

FEBS letters 20150128 5

Glutathione carries out vital protective roles within mitochondria, but is synthesised in the cytosol. Previous studies have suggested that the mitochondrial dicarboxylate and 2-oxoglutarate carriers were responsible for glutathione uptake. We set out to characterise the putative glutathione transport by using fused membrane vesicles of Lactococcus lactis overexpressing the dicarboxylate and 2-oxoglutarate carriers. Although transport of the canonical substrates could be measured readily, an exc ...[more]

PMID: 25637873

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