Testing tuberculosis drug efficacy in a zebrafish high-throughput translational medicine screen.

ABSTRACT: The translational value of zebrafish high-throughput screens can be improved when more knowledge is available on uptake characteristics of potential drugs. We investigated reference antibiotics and 15 preclinical compounds in a translational zebrafish-rodent screening system for tuberculosis. As a major advance, we have developed a new tool for testing drug uptake in the zebrafish model. This is important, because despite the many applications of assessing drug efficacy in zebrafish research, the current methods for measuring uptake using mass spectrometry do not take into account the possible adherence of drugs to the larval surface. Our approach combines nanoliter sampling from the yolk using a microneedle, followed by mass spectrometric analysis. To date, no single physicochemical prope

SUBMITTER: Ordas A 

PROVIDER: S-EPMC4335901 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature