DNA nanoswitches: a quantitative platform for gel-based biomolecular interaction analysis.

ABSTRACT: We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions using a gel electrophoresis readout. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrated this low-cost, versatile, 'lab-on-a-molecule' system by characterizing ten different interactions, including a complex four-body interaction with five discernible states.

SUBMITTER: Koussa MA 

PROVIDER: S-EPMC4336243 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature